cle casp 8 Search Results


cleaved caspase 3  (Proteintech)
96
Proteintech cleaved caspase 3
Statins <t>induce</t> <t>caspase-3</t> cleavage and upregulate CASP3 transcription in colorectal cancer cells. ( A ) Representative Western blots showing expression of cleaved caspase-3 (p17, 17 kDa) and GAPDH (loading control) in HT29 cells treated with LPS alone or LPS in combination with atorvastatin (20, 50 μg/mL) or rosuvastatin (10, 20 μg/mL) for 24 h; ( B ) Densitometric quantification of cleaved caspase-3 expression normalized to GAPDH in HT29 cells; ( C ) RT-qPCR analysis of CASP3 mRNA expression in HT29 cells, normalized to GAPDH and expressed as fold change relative to untreated controls; ( D ) Representative Western blots of cleaved caspase-3 and GAPDH in Caco-2 cells under identical treatment conditions; ( E ) Densitometric quantification of cleaved caspase-3 expression normalized to GAPDH in Caco-2 cells; ( F ) RT-qPCR analysis of CASP3 mRNA expression in Caco-2 cells. Data are presented as the mean ± SEM from three independent experiments. Statistical significance was determined by one-way ANOVA followed by Dunnett’s post hoc test. * p < 0.05, ** p < 0.01 versus LPS-treated control; ns, not significant.
Cleaved Caspase 3, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cle+casp+8/Caspase+8%2Fp43%2Fp18+Antibody/pmc12841271-465-55-58
Average 96 stars, based on 1 article reviews
cleaved caspase 3 - by Bioz Stars, 2026-10
96/100 stars
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cleaved caspase 3  (Cell Signaling Technology Inc)
99
Cell Signaling Technology Inc cleaved caspase 3
Statins <t>induce</t> <t>caspase-3</t> cleavage and upregulate CASP3 transcription in colorectal cancer cells. ( A ) Representative Western blots showing expression of cleaved caspase-3 (p17, 17 kDa) and GAPDH (loading control) in HT29 cells treated with LPS alone or LPS in combination with atorvastatin (20, 50 μg/mL) or rosuvastatin (10, 20 μg/mL) for 24 h; ( B ) Densitometric quantification of cleaved caspase-3 expression normalized to GAPDH in HT29 cells; ( C ) RT-qPCR analysis of CASP3 mRNA expression in HT29 cells, normalized to GAPDH and expressed as fold change relative to untreated controls; ( D ) Representative Western blots of cleaved caspase-3 and GAPDH in Caco-2 cells under identical treatment conditions; ( E ) Densitometric quantification of cleaved caspase-3 expression normalized to GAPDH in Caco-2 cells; ( F ) RT-qPCR analysis of CASP3 mRNA expression in Caco-2 cells. Data are presented as the mean ± SEM from three independent experiments. Statistical significance was determined by one-way ANOVA followed by Dunnett’s post hoc test. * p < 0.05, ** p < 0.01 versus LPS-treated control; ns, not significant.
Cleaved Caspase 3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cle+casp+8/Cleaved+Caspase-3+(Asp175)+Antibody/pmc09166592-72-62-66
Average 99 stars, based on 1 article reviews
cleaved caspase 3 - by Bioz Stars, 2026-10
99/100 stars
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elisa kit cleaved casp8 human  (G Biosciences)
N/A
An ELISA kit for the detection of Cleaved CASP8 Human This uses Sandwich ELISA Double Antibody and has a sensitivity of 0 019ng ml
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cleaved casp8 d384 antibody  (Abbexa)
N/A
Cleaved CASP8 D384 Antibody is a Rabbit Polyclonal against Cleaved CASP8 D384
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cleaved-caspase 8 antibody (ya792  (MedChemExpress)
N/A
Cleaved-Caspase 8 Antibody (YA792) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Cleaved-Caspase 8.
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caspase 8 casp8 nm 001080125 human tagged orf clone lentiviral particle  (OriGene)
N/A
Lenti ORF particles CASP8 Myc DDK tagged Human caspase 8 apoptosis related cysteine peptidase CASP8 transcript variant G 200ul 10 7 TU mL
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caspase 8 casp8 nm 033358 human tagged orf clone lentiviral particle  (OriGene)
N/A
Lenti ORF particles CASP8 Myc DDK tagged Human caspase 8 apoptosis related cysteine peptidase CASP8 transcript variant E 200ul 10 7 TU mL
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flash casp8ap2 nm 012115 human tagged orf clone lentiviral particle  (OriGene)
N/A
Lenti ORF particles CASP8AP2 Myc DDK tagged Human caspase 8 associated protein 2 CASP8AP2 transcript variant 1 200ul 10 7 TU mL
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caspase 8 casp8 nm 001228 human tagged orf clone lentiviral particle  (OriGene)
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Lenti ORF particles CASP8 mGFP tagged Human caspase 8 apoptosis related cysteine peptidase CASP8 transcript variant A 200ul 10 7 TU mL
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caspase 8 casp8 nm 001080124 human tagged orf clone lentiviral particle  (OriGene)
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Lenti ORF particles CASP8 Myc DDK tagged Human caspase 8 apoptosis related cysteine peptidase CASP8 transcript variant F 200ul 10 7 TU mL
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caspase 8 casp8 nm 033356 human tagged orf clone lentiviral particle  (OriGene)
N/A
Lenti ORF particles CASP8 Myc DDK tagged Human caspase 8 apoptosis related cysteine peptidase CASP8 transcript variant C 200ul 10 7 TU mL
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Image Search Results


Statins induce caspase-3 cleavage and upregulate CASP3 transcription in colorectal cancer cells. ( A ) Representative Western blots showing expression of cleaved caspase-3 (p17, 17 kDa) and GAPDH (loading control) in HT29 cells treated with LPS alone or LPS in combination with atorvastatin (20, 50 μg/mL) or rosuvastatin (10, 20 μg/mL) for 24 h; ( B ) Densitometric quantification of cleaved caspase-3 expression normalized to GAPDH in HT29 cells; ( C ) RT-qPCR analysis of CASP3 mRNA expression in HT29 cells, normalized to GAPDH and expressed as fold change relative to untreated controls; ( D ) Representative Western blots of cleaved caspase-3 and GAPDH in Caco-2 cells under identical treatment conditions; ( E ) Densitometric quantification of cleaved caspase-3 expression normalized to GAPDH in Caco-2 cells; ( F ) RT-qPCR analysis of CASP3 mRNA expression in Caco-2 cells. Data are presented as the mean ± SEM from three independent experiments. Statistical significance was determined by one-way ANOVA followed by Dunnett’s post hoc test. * p < 0.05, ** p < 0.01 versus LPS-treated control; ns, not significant.

Journal: International Journal of Molecular Sciences

Article Title: Pharmacologic Modulation of the PAR-2–ERK Axis by Statins Converts Inflammatory Survival Signalling into Apoptosis in Colorectal Cancer Cells

doi: 10.3390/ijms27020916

Figure Lengend Snippet: Statins induce caspase-3 cleavage and upregulate CASP3 transcription in colorectal cancer cells. ( A ) Representative Western blots showing expression of cleaved caspase-3 (p17, 17 kDa) and GAPDH (loading control) in HT29 cells treated with LPS alone or LPS in combination with atorvastatin (20, 50 μg/mL) or rosuvastatin (10, 20 μg/mL) for 24 h; ( B ) Densitometric quantification of cleaved caspase-3 expression normalized to GAPDH in HT29 cells; ( C ) RT-qPCR analysis of CASP3 mRNA expression in HT29 cells, normalized to GAPDH and expressed as fold change relative to untreated controls; ( D ) Representative Western blots of cleaved caspase-3 and GAPDH in Caco-2 cells under identical treatment conditions; ( E ) Densitometric quantification of cleaved caspase-3 expression normalized to GAPDH in Caco-2 cells; ( F ) RT-qPCR analysis of CASP3 mRNA expression in Caco-2 cells. Data are presented as the mean ± SEM from three independent experiments. Statistical significance was determined by one-way ANOVA followed by Dunnett’s post hoc test. * p < 0.05, ** p < 0.01 versus LPS-treated control; ns, not significant.

Article Snippet: Membranes were blocked for 1 h at 4 °C in 3% bovine serum albumin (BSA) prepared in Tris-buffered saline (TBS), followed by overnight incubation at 4 °C with the following primary antibodies: PAR-2 (6976T, Cell Signaling Technology, Danvers, MA, USA), PAR-1 (79109T, Cell Signaling), total ERK1/2 (83533-1-RR, Proteintech, Minneapolis, MN, USA), phospho-ERK1/2 (Thr202/Tyr204) (20582-1-AP, Proteintech), cleaved caspase-3 (25128-1-AP, Proteintech), pro-caspase-8 (13423-1-AP, Proteintech), cleaved caspase-8 (9496T, Cell Signaling), BCL-2 (3498T, Cell Signaling), BAX (50599-2-Ig, Proteintech), and GAPDH (2118T, Cell Signaling), the latter used as the internal loading control.

Techniques: Western Blot, Expressing, Control, Quantitative RT-PCR